SC/-Ade/-Leu Broth - Names and Identifiers
SC/-Ade/-Leu Broth - Physico-chemical Properties
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SC/-Ade/-Leu Broth - Introduction
SC/-Ade/-Leu Broth(SC/-Ade/-Leu medium) is a commonly used laboratory medium for the cultivation of microbial cells. It is a synthetic medium that does not contain any natural nutrients and consists only of basic inorganic salts and a small amount of the required amino acids.
the main purpose of SC/-Ade/-Leu medium is to selectively cultivate yeast (Leu2 yeast) labeled with Leu2 active enzyme, and can also be used to study gene expression and gene complementation. This medium can provide the basic nutrients required for yeast growth and selectively promote the growth of Leu2 yeast through conditions lacking Leucine (leucine).
The method of preparing SC/-Ade/-Leu medium is to dissolve the synthetic medium powder in distilled water, adjust the pH, then add the required amino acids (Ade and Leu), and finally sterilize by high temperature and high pressure.
When using SC/-Ade/-Leu medium, care needs to be taken to comply with laboratory safety practices. Care should be taken to avoid contact with the culture medium skin, eyes and respiratory tract to prevent the occurrence of adverse reactions. In addition, follow proper disinfection procedures to prevent contamination of the culture medium.
Last Update:2024-04-09 19:06:00